Studies on the Human Placental ACTH-like Substances. II
Column Chromatography of Active Substances
1956 Volume 76 Issue 9 Pages 1038-1041
Details
1956 Volume 76 Issue 9 Pages 1038-1041
ACTH fraction (GE) of human placenta was submitted to column chromatography using oxycellulose or starch-succinic ester and the effective principle was found to have collected in the portion eluted with 0.01N hydrochloric acid solution, the portion eluted with 0.1N acetic acid being practically ineffective. Chromatography with starch-succinic ester afforded a fraction having about twice the effect of GF-fraction and its purity was found to be far higher than that of GF-fraction by paper elctrophoresis. However, this fraction was also found to be clearly different from ACTH in dose-response relationship, as was anterior lobe ACTH.
