Studies on Mercury Compounds of Dibromofluorescein. III

Chromatographic and Polarographic Researches on the Structure of Mercurochrome

Abstract

By adsorption chromatography using exsiccated aluminum hydroxide gel, components of mercurochrome were each isolated and their polarographic waves were examined. The conclusions reached agreed with results obtained in past experiments. Each of the fractions adsorbed is dissolved in aqueous sodium hydroxide solution, which contains a large amount of A1O₂- that will be useful as a supporting electrolyte, and this can directly be used as a polarographic electrolytic solution. This solution may be acidified and the aluminum hydroxide that precipitates out can be dissolved again to obtain the objective substance. This process offers a suggestion for the separatory purification and polarography of a complicated mixture.