Effects of Microbial Metabolites on the Activity of Cholinesterase. V

Experiments in various Fish Media

Abstract

Escherichia coli and Achoromobacter iophagum were incubated in a medium of a homogenate of 12 kinds of fish, crustaceae, and gastropods native to the Hokkaido. The pH of the culture filtrate, the amount of volatile basic nitrogen, trimethylaminenitrogen, and degree of cholinesterase inhibition were examined, as in the case of using squid as the medium.
Periodical variation of measured values was approximately the same as in the case of squid, while the trimethylamine-nitrogen and degree of cholinesterase inhibition. in the case of E. coli, were the greatest with squid, followed in the order of mackerel-pike, crab, octopus, shrimp, “Kinki”, and cod. With Ach. iophagum, the order was squid, shrimp, “Kinki”, horse-mackerel, Atka-mackerel, and crab. In general, gastropods and crustacea showed stronger inhibition of cholinesterase with microorganisms.