Studies on Tannins. VII

Colorimetric Determination of d-Catechin in Catechu

Abstract

Colorimetric determination of d-catechin with vanillin-hydrochloric acid reagent was carried out. Successful separation of d-catechin and Gambir tannin was effected by paper chromatography, from which d-catechin was eluted out with methanol and submitted to determination. By this means, sum of content of d-catechin and that of its optical isomers was obtained as expressed by d-catechin value.