Studies on Parotin-A.IV. : Effect of Parotin-A on Serum Citric Acid in Rabbits

Abstract

Effect of the intravenous injection of Parotin-A on serum citric acid in rabbits was investigated. Parotin (clinical use) was examined simultaneously in order to compare with the citric acid activity of Parotin-A. When the experiments were carried out using rabbits which were fed as usual, the variations of percentage decrease in serum citric acid were so large that the difference between the treated animals and controls injected with 0.9% saline solution was not statistically significant, especially when small doses were employed such as in this experiment. For that reason, the experiments were followed by use of the fasting animals. After fasting for 24 hours, the rabbits were injected with 0.1 mg. of Parotin-A per kg. body weight. The blood was drawn just before the injection and also 9 and 24 hours after injection, and serum citric acid was measured. In the case of 0.1 mg. administration only 9 hours' value differed significantly from that of the controls, and in the case of 0.3 mg. administration, both 9 and 24 hours' values were statistically significant. On the other hand, administration of 1.0 mg./kg. of Parotin (clinical use) caused significant decrease of serum citric acid only at 9 hours' value.