Gas-Liquid Chromatography of Alkaloids. V. : Gas Chromatographic Determination of Lycorenine

Abstract

In a previous paper, ³⁾ several alkaloids of Lycoris radiata HERB. were separated by gas chromatography using HI-EFF 8B as liquid phase, but lycorenine, one of the main bases, failed to be detected under various conditions. In this report, we give a description of conversion of lycorenine into various derivatives such as acetyl lycorenine, tetrahydrohomolycorine trimethylsilyl ether, diacetyl tetrahydrohomolycorine and deoxylycorenine ; these derivaties were chromatographed with OV-101, OV-17 and XE-60 column. An excellent separation of lycorenine was obtained by converting to tetrahydrohomolycorine TMS ether on 3% XE-60 column at 200°. We tried also to applicate this method to the extract of the alkaloids of Lycoris radiata HERB. Both lycorenine and homolycorine, in the extract, were converted to tetrahydrohomolycorine, which then was trimethylsilanized and chromatographed. The elution pattern is shown on Fig. 5. The quantitative determination of total tetrahydrohomolycorine was carried out using carbazole as internal standard. The amount of homolycorine was determined with a HI-EFF 8B column, while the approximate quantity of lycorenine was obtained by subtracting the amount of tetrahydrohomolycorine corresponding to homolycorine from the total tetrahydrohomolycorine.