Change of Quantity of Each Major Diterpenoid During Growth of Isodon trichocarpus KUDO Its Exploration by GC and GC-MS

Abstract

As a preliminary experiment of the biosynthesis, of changes in the quantity of major diterpenoids during growth of Isodon trichocarpus Kudo were examined gas chromatography and the combined gas chromatography-mass spectrometry (GC-MS). A change in the quantity was found by plotting the area ratio of trimethylsilylated diterpenoids to that of internal standard in gas chromatography every 10 days. Enmein and oridonin showed a similar tendency of changes in their quantity. They were found to increase markedly in June and July.