Studies on Doping Test by Gas Liquid Chromatography-Mass Spectrometry. II. : Detection and Identification of Antipyrine and Its Metabolites in Horse Urine (Analytical)

Abstract

When antipyrine is administered to the horse, unchanged antipyrine, 4-hydroxyantipyrine, and 3-hydroxymethyl-2-methyl-1-phenyl-3-pyrazolin-5-one are excreted in the urine. In the present study, a method was established to detect and identify these substances by thin-layer chromatography, gas chromatography, and gas chromatography-mass spectrometry.