Separation and Properties of a Low Molecular Weight Parotin-like Substance from Bovine Parotid Gland

Abstract

A parotin-like substance, fraction E-I, was separated in a highly purified form from the bovine parotid gland by mild procedures which comprised isoelectric fractionation, chromatography on CM-and ECTEOLA-cellulose. This substance produced hypocalcemia and leukocytosis preceded by transient leukopenia in rabbits by a single intravenous injection of 0.1 mg/kg. It also elicited depression of the serum acid phosphatase activity at a dose of 0.1 mg/kg. It has a molecular weight of 20000 and contains 33.00% hexose (galactose, glucose) and 18.89% glucosamine. The relation of this substance to other parotin-like substances from bovine parotid gland and human saliva is discussed.