Abstract
A new saccharogenic method is presented for the micro-determination of serum amylase activity, which uses only 5 μl of a sample. This is based on the determination of reducing sugar mixture produced in the enzyme reaction with insoluble starch substrate under conditions similar to that of the Somogyi method by means of micro-colorimetric technique for the determination of blood sugar with 3, 6-dinitrophthalic acid. The measured enzyme activity in this method can be converted into Somogyi units, by dividing it by 3, 66, which is commonly adopted in the saccharognic methods. The method gives reliable results, does not requure a standardized starch, and may be suitable for the micro-assay of enzyme in practical use.
