1977 Volume 97 Issue 1 Pages 1-4
It was found that 5-(3-tert-butylamino-2-hydroxypropoxy)-3, 4-dihydrocarbostyil hydrochloride (OPC-1085) fluoresced strongly when irradiated in HCl solution and that this fluorescent substance was 5-(3-tert-butylamino-2-hydroxypropoxy)carbostyril (dehydro-OPC-1085) by a spectroscotic methods. OPC-1085 can be determined by applying this fluorescence phenomenon. The determination of OPC-1085 in plasma was performed according to the following method. OPC-1085 was extracted from plasma with chloroform. The chloroform solution was divided into two equal parts, to each of which 1N HCl or 1N HBr solution was added. Each acid solution was irradiated by a tungsten lamp for 80 min. Difference in fluorescence intensity of the two solutions was measured at 460 nm, excited at 310 nm, since the fluorescence reaction is not observed in the HBr solution. The minimum determinable concentration was 0.1μg/ml in plasma.
