Antitumor Activity of Bacillus natto. VI. Analysis of Cytolytic Activity on Ehrlich Ascites Carcinoma Cells in the Culture Medium of Bacillus natto KMD 1126

Abstract

For the test of cytolytic activity on Ehrlich ascites carcinoma cells, a new method, named the sheet method, was employed. By means of the sheet method, it was proved that the culture filtrate of Bacillus natto KMD 1126 had a cytolytic activity on Ehrlich ascites carcinoma cells. As a result of the analysis of the cytolytic activity, surfactin (I), protease (II), and an acidic substance (III) were separated from the culture filtrate. I, II, and III had no cytolytic activity but a mixture of I, II, and III showed a cytolytic activity, same as that of the culture filtrate.