Simultaneous Determination of α-Tocopherol and α-Tocopheryl Acetate in Plasma by Mass Fragmentography

Abstract

A method for simultaneous analysis of α-tocopherol and α-tocopheryl acetate in plasma was investigated using massfragmentography for the purpose of biopharmaceutical studies of α-tocopheryl acetate administered parenterally to animals. α-Tocopherol and α-tocopheryl acetate in plasma were extracted with n-hexane, derivatized by silylation, and determined simultaneously by monitoring at m/e 502 for silylated α-tocopherol and at m/e 430 for α-tocopheryl acetate. This method was rapid and specific, and was satisfactorily employed in the biopharmaceutical studies of α-tocopheryl acetate.