1979 Volume 99 Issue 5 Pages 458-465
The antigenicity of some metabolites of sulfanilamide and their related compounds was studied by three kinds of experiments, 1) protein binding, 2) skin reaction in guinea pigs and 3) antibody formation to 4-hydroxylaminobenzenesulfonamide (4-HABSA). 1) The strong binding of 4-HABSA to bovine serum albumin (BSA) and rat serum protein was found by extensive equilibrium dialysis and gel filtration on a Sephadex G-25 column. 2) In addition, in both groups of guinea pigs sensitized with SA or 4-HABSA, positive skin reaction was observed 24 hr after elicitation by injections of 4-HABSA, 4-nitro-sobenzenesulfonamide (4-NSBSA), 4, 4'-azoxybenzenedisulfonamide (4, 4'-AZOBDSA), 4, 4'-azobenzenedisulfonamide (4, 4'-AZBDSA) and 4, 4'-hydrazobenzenedisulfonamide (4, 4'-HAZBDSA). 3) We also attempted to detect antibodies to 4-HABSA in the antisera of rabbits sensitized with 4-HABSA alone or 4-HABSA-BSA conjugate using passive hemagglutination tests. It is thought that 4-HABSA conjugated with tissue proteins acts as antigen, although the production of antibody to 4-HABSA hapten is low.
