Studies on Duboisia Species. III. Terpenoids (Ubiquinone and Triterpene) in Cultured Cells and Leaves of Duboisia leichhardtii

Abstract

Cullus tissues were induced from the shoot of Duboisia leichhardtii F. MUELL (Solanaceae) on R-2-V agar medium supplemented with casein (0.2%) and 6-benzyladenine (0.5 ppm). Cultured cells were obtained from R-2-V liquid medium without cytokinin. Terpenoids (ubiquinone and triterpenoids) in cultured cells and intact plant were examined and compared. Main ubiquinone in cultured cells and intact plant was identified as ubiquinone-10 by the high performance liquid chromatography method. Ubiquinone-10 content in cultured cells (50-80 μg/g dry wt.) was higher than in intact plant (12.6 μg/g dry wt.). Triterpenoids in cultured cells and medium were different from intact plant. Triterpenoids in cultured cells and medium were identified as lupeol and phytosterols, on the other hand, ursolic acid, oleanolic acid, 3-keto-oleanolic acid, acetylursolate, and phytosterols were obtained from intact plant.