High-Performance Liquid Chromatographic Determination of Acetohexamide and Its Metabolite, Hydroxyhexamide

Abstract

A simple HPLC assay was developed for acetohexamide and hydroxyhexamide, its active metabolite in plasma. Plasma sample was extracted with benzene-ethyl acetate at pH 5 and the organic phase was evaporated. The 50% acetonitril solution of the residue was chromatographed using Lichrosorb RP-8 reverse phase column and the mobile phase composed 0.2% AcOH-acetonitrile (1 : 1). This method can be used for bioavailability and clinical pharmacokinetic studies of acetohexamide.