Simultaneous Detection of Hepatitis B, C, and G Viral Genomes by Multiplex PCR Method

Nami Konomi; Mari Yamaguchi; Hideo Naito; Naoto Aiba; Takahide Saito; Yasuyuki Arakawa; Kenji Abe

doi:10.7883/yoken.JJID.2000.70

Short Communication

Simultaneous Detection of Hepatitis B, C, and G Viral Genomes by Multiplex PCR Method

Nami Konomi, Mari Yamaguchi, Hideo Naito, Naoto Aiba, Takahide Saito, Yasuyuki Arakawa, Kenji Abe

著者情報

ジャーナルフリー

2000 年 53 巻 2 号 p. 70-72

DOI https://doi.org/10.7883/yoken.JJID.2000.70

詳細

抄録

We established a multiplex polymerase chain reaction (PCR) method for simultaneous detection of hepatitis B, C, and G viral genomes. The levels of concordance with the data obtained by conventional single PCR method were 100% for single infection, 98 to 100% for double infections, and 92% for triple infections. This method is not only suited to rapid, large-scale epidemiological screening and clinical diagnosis of those virus infections occurring alone or in combination, but is also time- and cost-effective.

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