論文ID: JJID.2014.441
Dengue virus (DENV) infection is a serious global health threat. For dengue disease surveillance and control, there is a need for robust diagnostic tools, which are relatively easy to use and reliable in various clinical settings. We determined the utility of NS1 antigen detection in urine samples for DENV diagnosis using 118 urine samples obtained from 96 dengue patients at various phases of disease. NS1 antigen was detected by ELISA in urine samples of dengue patients obtained at days 2-17 after disease onset. Positive detection rates of NS1 antigen ranged from 13-43%. Using real-time RT-PCR, positive detection rates of viral genome in urine samples ranged from 20-33% on days 0 to ≥15. At days 11 to ≥15 after disease onset, NS1 antigen was detected at similar rates in serum and urine samples. Additionally, NS1 antigen was detected in 2 urine samples, but not in serum samples, on days 7 and 16 after disease onset. The results indicate the utility of NS1 antigen detection in urine samples using NS1 antigen ELISA to confirm acute DENV infection, and suggest that the assay is potentially useful when only limited amounts of serum samples are available, and in limited resource settings.
