論文ID: JJID.2017.078
Mutations in the reverse transcriptase (RT) region of the hepatitis B virus (HBV) genome are an important factor in reduced therapeutic effectiveness. However, the prevalence of these mutations in HBV strains isolated previously from Indonesia has not been systematically examined. Therefore, this study investigated the profile of mutations in the RT region and the associations of these mutations with amino acid changes in the surface protein in treatment-naïve Indonesian HBV carriers. Overall, 96 sequences of the full-length Indonesian HBV genome (genotype B, n=54; genotype C, n=42) were retrieved from the National Center for Biotechnology Information. Naturally occurring primary and/or compensatory drug resistance mutations were found in 6/54 (11.1%) genotype B strains and in 1/42 (2.4%) genotype C strains. The potential resistance against nucleos(t)ide analog and/or pretreatment mutations were more frequent in both genotypes but, the genotype C was much more than genotype B. The A–B interdomain region in the RT gene was more frequently mutated in genotype C than in genotype B (3.51±2.53 vs 1.08±1.52, P<0.001). Knowledge of the mutational profiles in RT gene and the changes in surface protein might help clinicians to select the most appropriate antiviral drug and vaccination/HBV immunoglobulin therapy for preventing HBV infection in Indonesia.
