The Japanese Medical Journal
Online ISSN : 1884-281X
ISSN-L : 0368-3095
THE COLORIMETRIC ESTIMATION OF FREE AND COMBINED CHOLESTEROL IN SERUM
FUMIMASA YANAGISAWAMASAKI MIZOKOSHI
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1950 年 3 巻 2 号 p. 137-140

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This paper describes a simple and quick method for determination of free and combined cholesterol in, serum. This method requires less hours to complete the determination as compared to the older method which requires at least twenty-hours. The total choleterol is first determined by Zuckerman's method (1) . Combined and free cholesterol are determined on the chloroform extract with the aid of digitonin. Free cholesterol is in the precipitates with digitonin and the combined cholesterol remains in solution. This method is quicker and more convenient than the method of Shoenheimer (2), Sperry (3), or Pijoan (4) .
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