STUDIES ON THE ACCESSORY FACTOR FOR TOXOPLASMA DYE TEST GUINEA PIG SERUM AS A SOURCE OF THE ACCESSORY FACTOR

Abstract

A study is presented of the successful application of guinea pig sera as a source of the accessory factor to the toxoplasma dye test. A total of 209 serum samples were obtained from guinea pigs of different ages from 10 to 185 days. The sera were tested for possible usefulness as an accessory factor with the aid of Alsever solution. Without addition of Alsever, all the sera deprived the toxoplasma cytoplasm of its affinity for methylene blue. To the contrary, when Alsever was added, approximately 20 % of the serum samples were proved to be useful as a source of the accessory factor. Three-fifths of these qualified sera produced titers higher by 1 tube or 4 fold for a positive control serum than that estimated by using a human accessory factor serum, while remaining two-fifths showed the same titer as that with the human serum.