Abstract
Three-dimensional (3D) NMR spectroscopy has been shown to be an effective method for resolving spectral overlap encountered in two-dimensional (2D) NMR experiments. We mainly explain heteronuclear three-dimensional experiments which are combinations of a heteronuclear shift correlation experiment with homonuclear 2D NMR experiments. Homonuclear 2D spectra (eg. DQF-COSY, HOHAHA) can be simplified with this technique by editing with respect to the heteronuclear chemical shift in a third-dimension. We introduce advantage, disadvantage and various practical aspects of three-dimensional NMR spectroscopies.
