Application to the Preparation of Useful Compounds Based on Enzyme-and Microorganism-mediated Reactions

Abstract

This article describes our recent studies on enzyme-and microorganism-mediated reactions, and application to the preparation of useful compounds based on the complementary chemo-enzymatic synthesis. First, lipase-catalyzed reactions were focused especially on the preparation of enantiomerically pure compounds, such as N-Boc-indoline-2-carboxylic acid, indanoxazolidinone and symbioramide. Next, the control of nitrile-hydrolyzing enzyme in Rhodococcus rhodochrous was summarized. A one-pot chemo-enzymatic transformation of aldehydes to amides was established. The third topic is the application of yeast-mediated asymmetric reduction of carbonyl compounds. The preparation of a couple of enantiomerically pure starting materials for natural product synthesis by the combination with chemical transformation was disclosed.