The purpose of our study was to investigate basic mechanisms of hydrogen (H
2) gas – induced cardioprotection during oxidative stress in a cardiomyocytes cell culture model. H
2 gas has been demonstrated to reduce the hydroxyl radical (•OH), the most cytotoxic of reactive oxygen species (ROS), and effectively protected cells. Here, we developed a new model for evaluating that H
2 gas has the potential to act as antioxidant on H
2O
2-induced death of H9c2 cell. Treatment with H
2O
2 (500µM) induced death of H9c2 cells. However, co-treatment with H
2 gas increases viability of cells. These results suggest that method is a useful alternative animal model to clarify the mechanism which H
2 gas protects H9c2 cardiomyocytes from oxidative stress.
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