Alternatives to Animal Testing and Experimentation
Online ISSN : 2185-4726
Print ISSN : 1344-0411
ISSN-L : 1344-0411
Volume 12 , Issue 3
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Review
Original Article
  • Takashi Omori, Takashi Sozu
    Type: Original Article
    2007 Volume 12 Issue 3 Pages 212-217
    Published: October 31, 2007
    Released: February 28, 2012
    JOURNALS FREE ACCESS
    The local lymph node assay (LLNA) is a well-established method for assessing skin sensitization; it involves the use of mice and provides an alternative to tests requiring guinea pigs. In this assay, the endpoint of interest is the stimulation index (SI) that is defined as the mean disintegrations per minute (DPM)/mouse for the chemical-treated group divided by that for the vehicle control group. Therefore, the SI is a ratio of 2 variables. However, some researchers often ignore the variation in the vehicle control group while assessing chemicals based on the SI. This approach, which we refer to as the ignorance approach, may underestimate the variation in SI. We developed an alternative approach by the delta method, and we evaluated the performance of both approaches through a simulation and a numerical examination. We found that the ignorance approach is not acceptable for estimating the variance of SI because the value obtained is severely biased toward underestimation. Instead, analysis by using the delta method approach is recommended. However, it should be noted that this approach is also slightly biased in the case of large variations in the DPM/mouse values in the vehicle control group.
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