The effect of sex ratio on the behavior and the duration of tonic immobility (TI) of broilers were investigated. Five variants of the sex ratio (male to female), namely, 100:0, 75:25, 50:50, 25:75 and 0:100 were tested in broiler chicks between the ages of 7 to 35 days. It was found that the most common behavior in all sex ratios was resting, ranging from 75.3 to 85.3%. The ranges of other behavior traits were as follows: pecking, 7.4 to 20.2% and moving, 1.3 to 11.5%. The behavioral frequency of pecking and resting were significantly affected by the age (P<0.05), but not affected by sex ratio. The percentage of moving behavioral frequency was significantly different by age (P<0.01) and sex ratios (P<0.05) with moving in 25:75 male to female ratio being higher than other sex ratios (P<0.05). The behavioral frequency of pecking, resting and moving were significantly interaction between age and sex ratio of broiler chickens (pecking and resting, P<0.05; moving, P<0.01). The duration of TI reaction was significantly affected by the age (P<0.01), sex (P<0.01), and sex ratio (P<0.05). A significantly interaction among age, sex and sex ratio was found. The TI reaction was significantly lower in 25:75 male to female ratio than other sex ratios on the both 19 and 33 age (P<0.05). There was a highly inverse relationship between moving behavioral frequency and TI reaction; moving abilities at 3 weeks and TI reaction at 19 days of age (Y=13.12X-0.07, r=-0.83); and moving abilities at 5 weeks and TI reaction at 33 days of age (Y=7.72X-0.31, r=-0.76), respectively. The results seems that, when male and female broiler chickens are reared together, locomotor abilities are affected at a male to female ratio of 25:75.
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