ACTA HISTOCHEMICA ET CYTOCHEMICA Vol. 50(2017) No. 3

REGULAR ARTICLE

The Effect of Ultraviolet B on Fibrillin-1 and Fibrillin-2 in Human Non-pigmented Ciliary Epithelial Cells In Vitro

Yuki Shiroto, Shingo Terashima, Yoichiro Hosokawa, Kyoko Oka, Keitaro Isokawa, Eichi Tsuruga

Released: June 27, 2017

[Advance Publication] Released: June 14, 2017

p.105-109

The ciliary zonules link the lens to the ciliary body in the eye, controlling the thickness of the lens for focusing through their characteristic elasticity. The ciliary zonules are composed of oxytalan fibers. Physiological or pathological damage to the ciliary zonules, including exposure to ultraviolet (UV)-A and UV-B components, can lead to lens dislocation. However, no studies have shown whether UV affects the ciliary zonule. Here, we assessed the effects of UV light on human nonpigmented ciliary epithelial cells (HNPCECs). HNPCECs were cultured for 4 weeks, and expression of fibrillin-1 and fibrillin-2 was confirmed. In control cultures (0 mJ/cm²), some fibrillin-1-positive fibers were merged with fibrillin-2. After UV-A irradiation, the appearance of both fibrillin-1- and fibrillin-2-positive fibers was unchanged. However, after UV-B irradiation, fibrillin-1-positive fibers became thin at an irradiation level of 100 mJ/cm², and the fiber structure became amorphous at 150 mJ/cm². Fibrillin-2-positive fibers lost their continuity and disappeared after being exposed to 150 mJ/cm² UV-B. UV-B irradiation did not affect cell viability, possibly because of the sensitivity of fibrillin-1 and fibrillin-2 to UV-B. Thus, dislocation of the lens with age may be attributable to cumulative exposure to UV-B.

Expression of c-kit and Cytokeratin 5 in the Submandibular Gland after Release of Long-Term Ligation of the Main Excretory Duct in Mice

Hiroshi Watanabe, Haruka Takahashi, Miyuki Hata-Kawakami, Akira Tanaka

Released: June 27, 2017

[Advance Publication] Released: June 22, 2017

p.111-118

Long-term submandibular duct obstruction is thought to cause irreversible atrophy and dysfunction of the submandibular gland. As an atrophic gland may be induced clinically by chronic or recurrent infection, it is generally removed surgically. However, the regenerative capacity of atrophic submandibular glands after long-term obstruction is not completely understood. We studied the regenerative capacity of the mouse submandibular duct using an aneurysm clip ligation model. We documented changes in the size, histologic structure and gene expression of the submandibular gland after 2 months of ligation, and 1 week, 1 month and 2 months after clip removal. Two months of ligation caused atrophy, particularly in the acinar portion. In the 2 months after clip removal, we observed a steady and significant increase in the expression of the acinar cell precursor gene cytokeratin 5 (CK5), and a significant decrease in the expression of the stem cell marker c-kit. These findings suggest that the submandibular gland retains some capacity for regeneration even after long-term obstruction, and that CK5 could serve as a marker of this regenerative process.

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Volume And Issue List
ACTA HISTOCHEMICA ET CYTOCHEMICA

Predecessor

ACTA HISTOCHEMICA ET CYTOCHEMICA Vol. 50(2017) No. 3

REGULAR ARTICLE

The Effect of Ultraviolet B on Fibrillin-1 and Fibrillin-2 in Human Non-pigmented Ciliary Epithelial Cells In Vitro

Expression of c-kit and Cytokeratin 5 in the Submandibular Gland after Release of Long-Term Ligation of the Main Excretory Duct in Mice

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Announcement From J-STAGE

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Volume And Issue List ACTA HISTOCHEMICA ET CYTOCHEMICA

Predecessor

ACTA HISTOCHEMICA ET CYTOCHEMICA Vol. 50(2017) No. 3

REGULAR ARTICLE

The Effect of Ultraviolet B on Fibrillin-1 and Fibrillin-2 in Human Non-pigmented Ciliary Epithelial Cells In Vitro

Expression of c-kit and Cytokeratin 5 in the Submandibular Gland after Release of Long-Term Ligation of the Main Excretory Duct in Mice

System Maintenance

Announcement From J-STAGE

Journal Tools

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Volume And Issue List
ACTA HISTOCHEMICA ET CYTOCHEMICA