Mating system of Meehania montis-koyae, a rare and endangered perennial herb, was studied by crossing experiments for multiple years and study sites. We conclude this species is self-fertile since both selfed and bagged flowers produced considerable fruits. Compare with other treatments (=selfing, crossing, and control), seed set of bagged flowers were significantly low. During the period of the observation the flowers of M. montis-koyae, visits of syrphus flies and small carpenter bees were confirmed but less frequently. Therefore, M. montis-koyae needs pollen vectors for seed production, however, there may be pollen limitation because of insufficient pollinator visits.
Eleocharis dulcis, Rumex longifolius and Persicaria erectominor were newly found from Shimane Pref. The Hii River in Izumo City revealed the western limit of the distribution of R. longifolius and the Lake Nakaumi is the second record of P. erectominor from the area along the Sea of Japan in Kinki-Chugoku District. Persicaria erectominor var. erectominor f. viridiflora showed some difference from f. erectominor on phenology, morphology and distribution.
Pyrola japonica Klenze ex Alefeld var. subaphylla (Maxim.) Andres, a myco-heterotrophic variety of P. japonica, was recorded in Shikoku District, Japan. Three individuals were observed in a deciduous forest in Naka-cho, Naka-gun, Tokushima Prefecture.