RNA prepared from liver of rats given 4-dimethylaminoazobenzene [
N-methyl-
14C] (N-methyl-
14C-DAB) 24 hours earlier was shown to be radioactive. The radioactivity was proportional to the specific activity of administered N-methyl-
14C-DAB, The RNA was subjected to acid or alkaline hydrolysis, followed by ion-exchange column chromatography. The radioactivity was detected only in purine bases (adenine> guanine). Absorption spectra of the RNA showed that aminoazo dye is not bound to any detectable extent. The acid treatment of the RNA did not liberate any radioactivity in a volatile form.
These observations indicate that RNA is neither modified with aminoazo dyes nor with formaldehyde derived from N-methyl of DAB in acid-labile form. The methylation of guanine as occurring in the case of dimethylnitrosamine was also denied by the result of ion-exchange chromatography of the acid hydrolysate. The radioactivity of RNA was interpreted as being due to the incorporation of
14C-labeled C
1-compounds (formaldehyde and/or formic acid) derived from
in vivo N-demethylation of the labeled DAB into adenine and guanine through the biosynthetic pathway, supporting the concept by Berenbom.
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