For the diagnosis of liver diseases, the determinations of alkaline phosphatase (AL-P) or 5′-nucleotidase (5′-N) and transaminase (GOT, GPT), glucose-6-phosphatase (G6Pase) or isocitrate dehydrogenase (ICDH) are most useful, as already well-known. The present work has been done for the elucidation of the background on which such clinical applications were based.
AL-P, 5′N and G6Pase were comprised as the same category of phosphomonoesterase, however, each substrate of each one is not the same respectively, i. e. isodynamic enzyme. Besides, as to AL-P and ICDH also some different enzymes, so-called isozyme, have been seen. Accordingly, to investigate the origins of these enzymes, some potent methods applied for the studies on isozyme must be of use, indeed.
Along these line, these enzymes have been investigated on the sera and hepatic tissues impaired of human, as well as of rats.
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