Journal of Oral Tissue Engineering
Online ISSN : 1880-0823
Print ISSN : 1348-9623
Volume 12 , Issue 2
Showing 1-7 articles out of 7 articles from the selected issue
ORIGINAL ARTICLES
  • Hiromasa MORITA, Nobuhiro SHIGEMATSU, Tomoo KONO, Makoto UMEDA
    Type: ORIGINAL ARTICLE
    2014 Volume 12 Issue 2 Pages 57-68
    Published: 2014
    Released: March 22, 2015
    JOURNALS FREE ACCESS
    Vascular endothelial growth factor (VEGF) has been recognized as closely related diabetic complications. In this study, we created periodontal defects in diabetic model rats and examined the effects of VEGF expressed in wound healing process on the circulating VEGF concentration.In type 2 diabetic model (GK) and normal (SD) rats, periodontal defects were prepared in the bilateral upper molar regions. The VEGF expression in the periodontium 3, 5, and 7 days after surgery was examined by immunohistochemical staining. In addition, circulating blood was collected from the right atrium, and the plasma VEGF concentration was determined by ELISA.VEGF was expressed more intensely around microvessels, and the plasma VEGF concentration was higher in GK rats than in the SD rats throughout the study.These results suggest that, after periodontal surgery in diabetic rats, VEGF expressed in the periodontium affects the circulating VEGF concentration.
    Download PDF (809K)
  • Momoe NANDO, Kazuya TOMINAGA, Tsurayuki TAKAHASHI, Akio TANAKA, Makoto ...
    Type: ORIGINAL ARTICLE
    2014 Volume 12 Issue 2 Pages 69-79
    Published: 2014
    Released: March 22, 2015
    JOURNALS FREE ACCESS
    To clarify the function of novel synthetic peptide with an amino acid sequence the same as a partial sequence of amelogenin gene exon 5, we applied them to periodontal tissue defects artificially prepared in the rat molar region. Sections of the applied regions were prepared 3, 5, 7, and 14 days after surgery and histopathologically investigated. In addition, type III collagen and von Willebrand factor were immunohistochemically observed. Epithelial cell growth was promoted for 3 days after surgery and then inhibited thereafter. Fibroblast growth was advanced, type III collagen decreased in the early phase, and the ratio of blood vessels significantly increased, showing the promotion of vascularization. The novel synthetic peptide seems to promote the wound-healing process, inducing periodontal tissue regeneration in the early phase.
    Download PDF (582K)
  • Nozomu FUKUI, Takaaki UENO, Yoshihiro KIMURA, Yoichiro NAKAJIMA, Akihi ...
    Type: ORIGINAL ARTICLE
    2014 Volume 12 Issue 2 Pages 80-84
    Published: 2014
    Released: March 22, 2015
    JOURNALS FREE ACCESS
    The aim of this study was to investigate the safety of use of platelet rich plasma gel (PRPG) during the healing process of extracted sockets of impacted mandibular third molar in humans.Ten patients were included in the present study. Impacted third molars were surgically extracted in the same session.The prepared PRPG was placed into the extracted sockets. We evaluated the wound after 7 days for postoperative redness, swelling, pus discharge, pain and the panoramic radiograph for changes in bone at the surgical site. Healing was uneventful in all the cases treated with PRPG. Panoramic radiographic examination after around 6 months did not show abnormal bone formation at the surgical site.The present study suggests that the usage of PRPG for impacted third molar extraction had no adverse effects and PRPG can be safely used in the healing process of extracted sockets.
    Download PDF (1767K)
  • Koichi IMAI, Kazuhiko SUESE, Yoshitomo HONDA, Tsubasa SHIRAI, Fumiya O ...
    Type: ORIGINAL ARTICLE
    2014 Volume 12 Issue 2 Pages 85-92
    Published: 2014
    Released: March 22, 2015
    JOURNALS FREE ACCESS
    Embryonic Stem Cell Test (EST) is an in vitro embryotoxicity screening test to predict human developmental toxicity, mainly used in Europe and the United States. In the present study, EL-M3 and ES-R1-EGFP B2/EGFP cell lines were used, instead of the mouse ES-D3 cell line that is used as an indicator of cell differentiation, to compare the developmental toxicities of 11 dental adhesives with those obtained with ES-D3 cells. The results were comparable between EL-M3 and ES-D3 cell lines. Specifically, all the products fall into the category of "non- embryotoxicity," although the results widely varied. Three-dimensional culture with glass fibers could also be employed for the experiment.
    Download PDF (950K)
  • Tomoharu OKAMURA, Tetsunari NISHIKAWA, Masahiro WATO, Kazuya TOMINAGA, ...
    Type: ORIGINAL ARTICLE
    2014 Volume 12 Issue 2 Pages 93-99
    Published: 2014
    Released: March 22, 2015
    JOURNALS FREE ACCESS
    The purpose of the present study is that attempting three-dimensional tissue culture of capillaries in vitro, using three kinds of porous scaffolds, coral, ceramic bone, and pumice stone. The proliferation of cells was enhanced time-dependently in the experimental group. 7, 14 and 21 days after addition of particles, the cell proliferation of the experimental group was significantly higher than that of the control group. In the surrounding region from the added pumice stone, capillary formation was low. Much cell proliferation and capillary formation was observed three-dimensionally around the coral and ceramic bone particles. In the coral additional group, the largest capillary width was observed in the anastomosis region of capillaries.
    Download PDF (926K)
REVIEW
  • Koichi IMAI
    Type: REVIEW
    2014 Volume 12 Issue 2 Pages 100-105
    Published: 2014
    Released: March 22, 2015
    JOURNALS FREE ACCESS
    The field of regenerative medicine has recently made marked advances in stem cell research. Adult stem cells, called somatic or tissue stem cells that can only be differentiated into limited cell types, have been generally used for regenerative medicine. However, with the development of iPS cells, tissues and cells of interest will be artificially generated and implanted into patients to repair sites of damage. In the present study, technical objectives for this purpose are summarized.
    Download PDF (1194K)
  • Koichi IMAI
    Type: REVIEW
    2014 Volume 12 Issue 2 Pages 106-110
    Published: 2014
    Released: March 22, 2015
    JOURNALS FREE ACCESS
    Development of new dental care has been strongly desired. This new pioneering technology must be innovative content. This time, some is not in the medical field, and then propose the following items with the aim of great development of the future of the dental office. 1) An automatic drug injection system that injects drugs into the body from a prosthesis in an emergency, 2) Intraoral treatment with phototoxicity-inducing substance, 3) Substitute for the eye using "Denture Camera", 4) Information system utilizing cavities in teeth and dentures.
    Download PDF (822K)
feedback
Top