Vascular endothelial growth factor (VEGF) has been recognized as closely related diabetic complications. In this study, we created periodontal defects in diabetic model rats and examined the effects of VEGF expressed in wound healing process on the circulating VEGF concentration.In type 2 diabetic model (GK) and normal (SD) rats, periodontal defects were prepared in the bilateral upper molar regions. The VEGF expression in the periodontium 3, 5, and 7 days after surgery was examined by immunohistochemical staining. In addition, circulating blood was collected from the right atrium, and the plasma VEGF concentration was determined by ELISA.VEGF was expressed more intensely around microvessels, and the plasma VEGF concentration was higher in GK rats than in the SD rats throughout the study.These results suggest that, after periodontal surgery in diabetic rats, VEGF expressed in the periodontium affects the circulating VEGF concentration.

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To clarify the function of novel synthetic peptide with an amino acid sequence the same as a partial sequence of amelogenin gene exon 5, we applied them to periodontal tissue defects artificially prepared in the rat molar region. Sections of the applied regions were prepared 3, 5, 7, and 14 days after surgery and histopathologically investigated. In addition, type III collagen and von Willebrand factor were immunohistochemically observed. Epithelial cell growth was promoted for 3 days after surgery and then inhibited thereafter. Fibroblast growth was advanced, type III collagen decreased in the early phase, and the ratio of blood vessels significantly increased, showing the promotion of vascularization. The novel synthetic peptide seems to promote the wound-healing process, inducing periodontal tissue regeneration in the early phase.

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The aim of this study was to investigate the safety of use of platelet rich plasma gel (PRPG) during the healing process of extracted sockets of impacted mandibular third molar in humans.Ten patients were included in the present study. Impacted third molars were surgically extracted in the same session.The prepared PRPG was placed into the extracted sockets. We evaluated the wound after 7 days for postoperative redness, swelling, pus discharge, pain and the panoramic radiograph for changes in bone at the surgical site. Healing was uneventful in all the cases treated with PRPG. Panoramic radiographic examination after around 6 months did not show abnormal bone formation at the surgical site.The present study suggests that the usage of PRPG for impacted third molar extraction had no adverse effects and PRPG can be safely used in the healing process of extracted sockets.

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Embryonic Stem Cell Test (EST) is an in vitro embryotoxicity screening test to predict human developmental toxicity, mainly used in Europe and the United States. In the present study, EL-M3 and ES-R1-EGFP B2/EGFP cell lines were used, instead of the mouse ES-D3 cell line that is used as an indicator of cell differentiation, to compare the developmental toxicities of 11 dental adhesives with those obtained with ES-D3 cells. The results were comparable between EL-M3 and ES-D3 cell lines. Specifically, all the products fall into the category of "non- embryotoxicity," although the results widely varied. Three-dimensional culture with glass fibers could also be employed for the experiment.

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The purpose of the present study is that attempting three-dimensional tissue culture of capillaries in vitro, using three kinds of porous scaffolds, coral, ceramic bone, and pumice stone. The proliferation of cells was enhanced time-dependently in the experimental group. 7, 14 and 21 days after addition of particles, the cell proliferation of the experimental group was significantly higher than that of the control group. In the surrounding region from the added pumice stone, capillary formation was low. Much cell proliferation and capillary formation was observed three-dimensionally around the coral and ceramic bone particles. In the coral additional group, the largest capillary width was observed in the anastomosis region of capillaries.

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The field of regenerative medicine has recently made marked advances in stem cell research. Adult stem cells, called somatic or tissue stem cells that can only be differentiated into limited cell types, have been generally used for regenerative medicine. However, with the development of iPS cells, tissues and cells of interest will be artificially generated and implanted into patients to repair sites of damage. In the present study, technical objectives for this purpose are summarized.

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Development of new dental care has been strongly desired. This new pioneering technology must be innovative content.　This time, some is not in the medical field, and then propose the following items with the aim of great development of the future of the dental office. 1) An automatic drug injection system that injects drugs into the body from a prosthesis in an emergency, 2) Intraoral treatment with phototoxicity-inducing substance, 3) Substitute for the eye using "Denture Camera", 4) Information system utilizing cavities in teeth and dentures.

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