Materials that inhibit dentin hypersensitivity include those that seal dentin tubules. The dentin tubule sealing properties of the materials should be strong and biocompatible. The newly developed dentin hypersensitivity suppressing material is a biomaterial that satisfies both of these properties. The main ingredient is calcium carbonate, which has applications in antacids (pharmaceuticals), bone replacement materials (medical devices), and food additives. In this study, we investigated some of the effects on dentin tubule sealing properties received from brushing and ultrasonic cleaning after dentin tubules were sealed by reacting the newly developed calcium carbonate with a phosphoric acid solution.