In order to shorten the period required to select sterile trees from the mating family of male-sterile
Cryptomeria japonica D. Don lines, germinated male-sterile
C.
japonica seeds were planted in sphagnum-filled vinyl pots (width, 15 cm; height, 20 cm) in early December and incubated at 23°C until the end of March in the following year. In early April, the pots were moved to the greenhouse. The seedlings in pots were treated with 100 ppm gibberellin in early July to accelerate their development of male flowers, which were collected from each seedling during the middle of December and examined under a microscope at 50 × magnification to detect the presence of pollen. Tree height, basal diameter, number of female flowers, the number of clusters of male flowers, and branch spread were also examined. The form and flowering characteristics of the seedlings depended on the number of seedlings planted per pot. When five or more were planted per pot the seedlings were small and some produced no male flowers. Furthermore, under these conditions, fertile male flowers were sometimes mistaken for sterile flowers. In contrast, in pots with fewer than four seedlings, all male-fertile seedlings produced fertile male flowers. Therefore, the time required to identify male-sterile seedlings from the mating family of male-sterile
Cryptomeria japonica D. Don lines can be shortened using the methods developed in this study.
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