In order to clarify the bacterial flora of elderly people with tube feeding and the factors affecting the bacterial flora, the bacterial flora in the palate, tongue, and pharynx of tube-fed people and people with oral intake were analyzed by next-generation sequencing analysis (NGS). Factors affecting the bacterial flora were examined using principal component analysis and correlation ratios. The subjects were 20 elderly people with tube feeding and 19 elderly people with oral intake. The age, gender, disease, and bedridden level were confirmed from the hospitalization records. The Japan Coma Scale, consciousness level, communication status, presence of remaining teeth, and CPI were evaluated by a dentist. Sampling was performed on the palate, tongue, and pharynx by the swab method. Next-generation sequencing was performed according to the conventional method, and the types and composition of bacteria were also evaluated. The Shannon index of the tube group in the palate, tongue, and pharynx were significantly lower than those of the oral intake group. The tube group had significantly more aerobic bacteria in the palate, tongue, and pharynx than the oral intake group. The facultative anaerobes of the tongue and pharynx in the tube group were significantly higher than those in the oral intake group. The bacterial composition of the palate, tongue, and pharynx in the tube group was predominantly the same : Neisseria, Streptococcus, and Rothia. Principal component analysis between the two groups showed that the contribution of the first principal component of the palate was 21.3%, 32.7% for the tongue, and 30.1% for the pharynx. In the palate, tongue, and pharynx, the correlation ratio of “tube/oral intake” was the highest. The correlation ratio of “tube/oral intake” in the palate was 0.423, the tongue was 0.517, and the pharynx was 0.518. The factor most closely associated with the palate, tongue, and pharynx flora was “tube/oral intake”. The flora of the palate, tongue, and pharynx in the tube feeding group had more predominantly aerobic bacteria and was low in diversity.
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