We developed InSpIon system capable of stable nanoESI spray. Here, K562 cell digests were measured by DDA-MS and DIA-MS with a typical open nanoESI spray system and a spray system inserted spray tip head into an ion transfer tube, and InSpIon system.

We evaluated the impact of surfactants on the recovery of dried peptides. Dried K562 cell digests were dissolved by solutions containing different concentrations of 12 surfactants and measured by DDA-MS and DIA-MS.

This data was utilized to assess the utility of perfusion culture in cultivating spheroids of sarcoma cells. The differences between spheroids cultured with perfusion and without perfusion were analysed through mass spectrometry. The spheroids were fabricated from NCC-UPS4-C1 cell line derived from a patient with undifferentiated pleomorphic sarcoma. We found the different protein expression levels between spheroids cultured with perfusion and without perfusion.