Japan Society for Clinical Proteomics (JSCP)

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The 1st JSCP Conference

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[title in Japanese]

Pages 1-
Published: 2005
Released on J-STAGE: June 22, 2019

DOIhttps://doi.org/10.14905/jscp.2005.0_1

CONFERENCE PROCEEDINGS FREE ACCESS

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Prologue

[title in Japanese]

Toshihide Nishimura

Pages 6-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_6_1

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Oral Presentation

[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Pages 6-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_6_2

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Differential display of proteins regulating colorectal carcinogenesis by isotope-coded affinity tagging (ICAT)

[in Japanese], [in Japanese], [in Japanese]

Pages 8-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_8_1

CONFERENCE PROCEEDINGS FREE ACCESS

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[title in Japanese]

[in Japanese]

Pages 8-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_8_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Pages 10-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_10_1

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[title in Japanese]

[in Japanese]

Pages 10-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_10_2

CONFERENCE PROCEEDINGS FREE ACCESS

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Pages 12-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_12_1

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Clinical proteome analysis by 2D-HPLC peptide MS profiling

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Pages 12-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_12_2

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Molecular biomarkers are biological molecules that are indicators of physiologic state and also of change during a disease process like cancer. Mass spectrometry-based protein/peptide expression as diagnostic biomarkers has been reported. To identify sensitive and accurate diagnostic biomarkers, differential peptide/protein profiling using blood is required. Here we established comprehensive and sensitive analytical platform using automated 2-dimensional LC-MALDI-TOF MS and identified several peptide biomarkers which discriminate liver caner and non-cancer disease. Serum samples were pretreated and peptides (Mr<10,000) fractions were obtained and separated by two-dimensional HPLC; strong cation exchange (SCX) for the 1st dimension and reverse phase (RP) for the 2nd dimension. The separated samples were spotted onto three MALDI targets automatically. More than 10,000 fractions were analyzed automatically by MALDI-TOF MS in reflector mode. Our system identified angiotensin II as a spike in processed serum at 1nM concentration. Huge data of the peptide expression profiles generated from mass spectrometry were analyzed by the differential analysis tools originally developed by us. Without re-purification of peptides, sequential analysis by MSn on MALDI-QIT-TOF MS revealed that some of these biomarkers were post-translationally modified. Analysis of human serum from liver cancer patients and non-cancer patients by our system revealed several differentially-expressed peptides. Our analytical platform for comprehensive differential peptidomics may useful for identification of the novel biomarkers which may improve the early diagnosis of cancer.

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Pages 14-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_14_1

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[title in Japanese]

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Pages 14-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_14_2

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[title in Japanese]

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Pages 16-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_16_1

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[title in Japanese]

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Pages 16-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_16_2

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[title in Japanese]

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Pages 18-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_18_1

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[title in Japanese]

[in Japanese], [in Japanese]

Pages 18-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_18_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Pages 20-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_20_1

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Pages 20-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_20_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Pages 22-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_22_1

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[title in Japanese]

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Pages 22-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_22_2

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[title in Japanese]

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Pages 24-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_24_1

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[title in Japanese]

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Pages 24-
Published: 2005
Released on J-STAGE: August 17, 2018

DOIhttps://doi.org/10.14905/jscp.2005.0_24_2

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