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Kazuyuki Nakamura
Session ID: S-1
Published: 2007
Released on J-STAGE: December 18, 2007
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We performed proteomic profiling of cancerous and paired non-cancerous tissues from Japanese HCC patients with HCV infection but not with hepatitis B virus infection or chronic alcoholic hepatitis by two-dimensional gel electrophoresis(2DE), tandem mass spectrometry(MS/MS), immunoblotting and immunohistochemical analysis. In the cancerous tissues HSP70 family proteins such as GRP78, HSC70, GRP75 and HSP70.1, HSP60, glutamine synthetase (GS), alpha-enolase (ENOA), phosphoglycerate mutase-1, triose phosphate isomerase and ATP synthase beta chain were increased two-fold more than in non-cancerous tissues. On the contrary almumin, ferritin light chain, smoothelin, tropomyosin beta chain, arginase 1, aldolase B, ketohexokinase and enoyl-CoA hydratase were decreased less than a half. Further examinations indicated that GS isoforms were much increased in highly differentiated HCC, and ENOA increased in poorly differentiated HCC which correlated positively with tumor size and venous invasion. We also performed PROTEOMEX in which antigenic proteins in cancerous tissues could be detected by 2DE-immunoblot and MS/MS analysis with autoantibodies in sera from HCC patients nad from healthy control. The candidates for the antigenic proteins were determined to be HSP70, peroxiredoxin and MnSOD. From these results we will discuss on possible molecular mechanisms for hepatocarcinogenesis by HCV infection and potential use of those proteins for clinical diagnosis of HCC in early stage and for immunotherapy of HCC.
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Takeshi Tomonaga, Yoshio Kodera, Keishi Umemura, Masahiko Nezu, Fumio ...
Session ID: S-2
Published: 2007
Released on J-STAGE: December 18, 2007
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Toyofumi Nakanishi
Session ID: S-3
Published: 2007
Released on J-STAGE: December 18, 2007
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Masaya Ono, Ayako Negishi, Setsuo Hirohashi, Tesshi Yamada
Session ID: S-4
Published: 2007
Released on J-STAGE: December 18, 2007
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Masamichi Oh-Ishi, Yoshihito Nii, Hiroshi Okusa, Tetsuo Fujita, Masats ...
Session ID: S-5
Published: 2007
Released on J-STAGE: December 18, 2007
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Hisashi Hirano, Noriaki Arakawa, Hiroshi Kawasaki, Eri Takahashi, Yusu ...
Session ID: S-6
Published: 2007
Released on J-STAGE: December 18, 2007
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Yoshitsugu Seo, Tetsuya Okano, Shinji Abe, Yoshinobu Saito, Jiro Usuki ...
Session ID: 1
Published: 2007
Released on J-STAGE: December 18, 2007
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Hideaki Mizutani, Akihiko Genma, Yuji Minegisi, Akihiko Miyanaga, Jyun ...
Session ID: 2
Published: 2007
Released on J-STAGE: December 18, 2007
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Koichi Yoshida, Toshihide Nishimura, Tomoyo Nakano, Ryutaro Nishiyama, ...
Session ID: 3
Published: 2007
Released on J-STAGE: December 18, 2007
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Masachika Hayashi, Akira Yamagata, Ken Oofusa, Toshinori Takada, Tomok ...
Session ID: 4
Published: 2007
Released on J-STAGE: December 18, 2007
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Michiyo Tsuru, Kensei Nagata, Michio Sata, Kei Matsuoka, Hideaki Yaman ...
Session ID: 5
Published: 2007
Released on J-STAGE: December 18, 2007
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This protein shows below important significance to become biomarker of bone metastasis.
1. Preparation of the patient to measure is serum.
2. Measurement time is short.
3. This protein recognized earlier than bone scintigraphy.
This protein shows below important clinical significance to bone metastasis.
1. Prevention of bone metastasis is enabled.
2. We can make inhibitor of this protein. Expectation to pharmaceuticals for prevention of bone metastasis.
3. The pain of bone metastasis will be gone eternally.
4. Macrobiotic expectation of the cancer patients.
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Yoko Tabushi, Tohru Takeuchi, Toyofumi Nakanishi, Takayuki Takubo
Session ID: 6
Published: 2007
Released on J-STAGE: December 18, 2007
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Hiroyasu Akatsu, Norihiro Ogawa, Katsuyoshi Mizukami, Takashi Ishii, H ...
Session ID: 7
Published: 2007
Released on J-STAGE: December 18, 2007
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Koh Nakata, Masachika Hayashi, Akira Yamagata, Ken Oofusa, Bruce Trapn ...
Session ID: 8
Published: 2007
Released on J-STAGE: December 18, 2007
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Yasuhito Abe
Session ID: 9
Published: 2007
Released on J-STAGE: December 18, 2007
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Hiroko Odani, Yoshiyuki Hiki, Hitoo Iwase, Sachiko Shimozato, Kazuo Ta ...
Session ID: 10
Published: 2007
Released on J-STAGE: December 18, 2007
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Satoru Kikuchi, Kazufumi Honda, Yasushi Handa, Hidenori Kato, Kohi Yam ...
Session ID: 11
Published: 2007
Released on J-STAGE: December 18, 2007
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Takao Kawakami, Atsushi Ogiwara, Harubumi Kato
Session ID: 12
Published: 2007
Released on J-STAGE: December 18, 2007
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Tetsuya Okano, Akihiko Gemma, Akira Takemura, Masahiko Shibuya, Kuniko ...
Session ID: 13
Published: 2007
Released on J-STAGE: December 18, 2007
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Tomohiko Hara, Kazufumi Honda, Miki Shitashige, Masaya Ono, Hideyasu M ...
Session ID: 14
Published: 2007
Released on J-STAGE: December 18, 2007
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Background: Actinin-4 was originally identified as an actin-binding protein associated with cell motility and cancer invasion and metastasis. However, actinin-4 is speculated to exert several distinct functions. Method and Result: The expression level of actinin-4 of prostate cancer cell lines (22RV1, PC-3 and LNCaP) were all decreased compared to that of normal prostate epithelial cells (PrEC) by Western blotting. The expression level of actinin-4 was significantly decreased in prostate cancer cells compared to that of non-cancerous basal cells in surgical prostate tissue by immunohistochemistry. Restoration of actinin-4 expression significantly inhibited its cell proliferation of 22RV1 in colony formation assay. Immunoprecipitation and MS analysis revealed that actinin-4 makes native complexes with several partner proteins in 22RV1 cells, including actin, clathrin heavy chain etc.. Clathrin is a coat protein that covers the internalized membrane pit and plays a central role in early endocytosis. We found the other endocytosis-related proteins interacted with actinin-4. Immunofluorescence microscopy revealed that dynamin and clathrin were co-localized with actinin-4 at dorsal sites of membrane ruffling, and the transfection of actinin-4 cDNA facilitated the transport of transferrin into peri-nuclear endosomes. We identified a panel of proteins whose expression and/or subcellular localization was regulated by actinin-4 using organelle fractionation and ICAT-MS. Conclusion: Disturbance of actinin-4-mediated endocytosis may be involved in prostate carcinogenesis.
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Takaki Hiwasa, Hideaki Shimada, Akiko Kagaya, Masaki Takiguchi, Takeno ...
Session ID: 15
Published: 2007
Released on J-STAGE: December 18, 2007
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Kazuko Matsumoto, Hideyuki Yokote, Mari Maegawa, Kaoru Tanaka, Yoshihi ...
Session ID: 16
Published: 2007
Released on J-STAGE: December 18, 2007
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