The paper reports on underwater shock waves application for modification and possible control of embryonic stem cell differentiation and proliferation in regenerative medicine. Underwater shock waves have been of interest for various scientific, industrial, and medical applications. In the present study shock waves were generated by electric discharge using a magnetic pulse compression circuit (MPC) and tungsten point to point electrodes. Underwater shock waves were applied to embryos of medaka through an experimental setup. A variety range of number of shock waves from 1 to 50 shots and with different overpressures was applied. After shock wave application, a clear modification was observed and the extents of effects were studied.

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Despite the great potential of sonoporation as a promising drug delivery technique in medicine, its applications have been limited mostly by the lack of understanding the underlying biophysical mechanism. We introduced a new method for capturing real time highly magnified images of cell-microbubble interaction and present an analytical analysis of the interaction.

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