Screening of good subjects is important for the study on non-contact healing. Results of quantitative measurement for healing abilities, we should consider amount of practice years and patients, and numbers of techniques of healers when screening tests. Mental or spiritual factors were not important relatively. Physical factors and the degree of skill were more important.

If we injure plants such as cucumber, the wound emits many biophotons. We have applied this phenomenon to measure effects of non-contact healing so-called as laying-on-of-hands. Our studies suggest that natural biophotons from injured cucumber is generated by plural biochemical reactions. In the present study, we discussed possible mechanisms ; 1) oxidation reaction of ascorbic acid, 2) biosynthesis of C9/C6 aldehyde and alcohol (so-called green order), 3) other defense reactions. We gave alpha-linolenic acid, linoleic acid, ascorbate oxidase (ASOD) and lipoxygenase (LOX) to pieces of cucumber. Results, those components increased the intensity of biophotons emitted from cucumber.