-
Takuma MIYATA, Saya KAWAI, Seri SEKI, Hiroki SHIBUTANI, Tomonari SHIBU ...
論文ID: 25-0233
発行日: 2025年
[早期公開] 公開日: 2025/09/11
ジャーナル
オープンアクセス
早期公開
Oxidative stress has attracted attention as an indicator of exercise load. Minimizing the impact on the body is essential during underwater treadmill exercise. Here, we conducted an exploratory study of the effects of dermal suction, which has been reported to improve blood flow in healthy dogs, prior to underwater treadmill exercise. Five healthy beagles (two neutered males and three spayed females) underwent dermal suctioning with Medicell for PETS over the entire body for 30 min. Underwater treadmill exercise was performed (water temperature: 32–33°C, water level: hip joint, load: 1.5 km/hr for 20 min). Blood samples were collected immediately before; immediately after; and 1 hr, 1 day, and 3 days after the underwater treadmill exercise; the diacron-reactive oxygen metabolites (d-ROMs), indicating the oxidation level, and biological antioxidant potential (BAP), indicating antioxidant capacity, were measured to determine the BAP/d-ROM ratio as the potential antioxidant capacity. The results showed that dermal suction significantly decreased d-ROMs 3 days after underwater treadmill exercise, and the BAP/d-ROM ratio was significantly higher immediately after exercise and 1 and 3 days after dermal suctioning. The parasympathetic nervous system is activated by dermal suctioning, resulting in muscle relaxation and mild psychological change. This study showed that dermal suction tended to decrease oxidative stress after exercise. Future studies should consider effects on the nervous system and exercise intensity indices and should be conducted in larger canine cohorts.
抄録全体を表示
-
Atsushi NISHIKAWA, Keito TSUNODA, Kaito HATANO, Yoshiaki TABUCHI, Tets ...
論文ID: 25-0273
発行日: 2025年
[早期公開] 公開日: 2025/09/11
ジャーナル
オープンアクセス
早期公開
Local anesthetics such as lidocaine have been used in humans and other animals to perform surgical procedures, therapeutics, and experiments. Lidocaine discarded into the environment through industrial waste, human and animal excretion, and household waste has been detected in the aquatic environment. For example, lidocaine in rivers, lakes, and influent and effluent water has been detected at wastewater treatment plants (7 ng/L–2.06 μg/L), suggesting that this chemical substance influences aquatic animals. However, the toxic effects of lidocaine on marine organisms have not been investigated. This study examined the acute toxicity of local anesthetics (lidocaine and dibucaine) on the marine zoea larvae of red-clawed crabs, which feature a life cycle in which adults live on land and larvae live in the sea. Survival analysis of zoea larvae revealed that dibucaine was more toxic than lidocaine. The 50% effective concentrations (EC50s) for lidocaine and dibucaine were 1.64 × 10−3 and 4.63 × 10−5 M, respectively, after 24 hr of exposure. Next, RNA sequencing was performed using the EC50 of each drug. Both local anesthetics were highly toxic to the kidneys, in addition to their effects on the heart, nerves, and liver. These local anesthetics were also found to affect melatonin metabolism and possibly decrease melatonin levels in zoea larvae. To the best of our knowledge, no prior study conducted a comprehensive analysis of the effects of local anesthetics on aquatic larvae, illustrating the need to focus on the toxicity of these drugs in aquatic animals.
抄録全体を表示
-
Shingo YAMAZAKI, Julia KINUYAMA, Hisako KANEDA, Noriyasu SASAKI, Takeh ...
論文ID: 25-0257
発行日: 2025年
[早期公開] 公開日: 2025/09/08
ジャーナル
オープンアクセス
早期公開
This study investigated the effects of soy isoflavone yeast fermented extract (soyF) and soy isoflavone yeast unfermented extract (soyN) on rat ileal smooth muscle contraction. SoyF and soyN inhibited carbachol (CCh)- or KCl-induced contraction in a concentration-dependent manner; however, these effects were stronger for CCh-induced contraction than that for KCl, and the relaxation effect was stronger for soyF than for soyN. SoyF-induced relaxation was attenuated by 4-aminopyridine (4-AP), a Kv channel inhibitor, and iberiotoxin (IbTX), a calcium-activated potassium channel (BK channel) inhibitor. This relaxation was also decreased by 9-(tetrahydrofuran-2-yl)-9h-purin-6-amine (SQ22536) and 1H-[1,2,4]oxadiazolo[4,3-a] quinoxalin-1-one (ODQ). Moreover, simultaneous treatment involving SQ22536 with 4-AP or IbTX further restored soyF-induced inhibition compared with each single treatment, whereas simultaneous treatment with ODQ and 4-AP or IbTX did not restore soyF-induced inhibition. SoyF increased cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) levels in ileal strips. In β-escin-permeabilized muscle, CCh, guanosine 5′-O-(3-thiotriphosphate) (GTPγS), and phorbol-12,13-dibutyrate ( PDBu)-enhanced Ca2+-induced contraction, but soyF suppressed this Ca2+ sensitization. In summary, yeast-fermented treatment increased soy isoflavone-induced inhibition of ileal smooth muscle contraction. These data suggest that soyF-induced relaxation was due to increasing cAMP and cGMP related to activating adenylate cyclase and guanylate cyclase. Moreover, it was demonstrated that soyF-induced relaxation is due to cell membrane hyperpolarization and inhibition of contractile proteins. Cell membrane hyperpolarization is probably mediated by the activation of Kv and BK channels via cGMP-related signals. Furthermore, the inhibitory effect on the contractile protein is due to Ca2+ desensitization, probably through suppression of the Rho kinase/myosin phosphatase targeting subunit (MYPT) and/or the PKC/CPI-17 pathway.
抄録全体を表示
-
Osamu TAIRA, Akira FUJIWARA, Ryosuke TAKAI, Nobuyuki TSUTSUMI, Katsuak ...
論文ID: 25-0279
発行日: 2025年
[早期公開] 公開日: 2025/09/08
ジャーナル
オープンアクセス
早期公開
The status of co-infection with porcine reproductive and respiratory syndrome virus type 1 (PRRSV-1) and type 2 (PRRSV-2) in Japan is poorly understood. A case of such co-infection was identified on a PRRSV-1 non-vaccinated farm in Kagoshima prefecture. Both PRRSV-1 and PRRSV-2 genomes were simultaneously detected in pig samples by RT-PCR, and molecular analysis confirmed PRRSV-1/PRRSV-2 co-infection in individual piglets. The PRRSV-1 strain (020-P4-EU) was classified as lineage 1 (sublineage: L1.2), showing high similarity to the Unistrain® PRRS vaccine and a Korean strain. The PRRSV-2 strain belonged to lineage 4 (Cluster III). These findings provide molecular evidence of PRRSV-1/PRRSV-2 co-circulation in Japan, suggesting the complex epidemiology of PRRSV in this region.
抄録全体を表示
-
Aki FUJIWARA-IGARASHI, Sho GOTO, Yuta NAKAZAWA, Takafumi OHSIMA, Haruy ...
論文ID: 25-0254
発行日: 2025年
[早期公開] 公開日: 2025/09/05
ジャーナル
オープンアクセス
早期公開
Information on inflammatory laryngeal masses in dogs remains extremely limited. We aimed to describe the clinical and histopathological features and outcomes of five dogs with bilateral, movable inflammatory laryngeal masses. Stridor was a common clinical sign, followed by dysphonia and snoring, all of which were mild. The presence of movable masses was confirmed in all dogs through fluoroscopic or echolaryngographic evaluation. Laryngeal endoscopy identified two masses in each dog, consistent with everted laryngeal saccules. Surgical excision was performed in all dogs, and no recurrence was observed during a median follow-up of 13 months. Histopathological analysis revealed chronic laryngitis characterized by fibrosis, lymphoplasmacytic infiltration, edema, and lymphangiectasia, consistent with findings reported in laryngeal saccules associated with brachycephalic airway syndrome in dogs.
抄録全体を表示
-
Jeongtae KIM, Kyungsook JUNG, Irene ORTIZ-LEAL, Pablo SANCHEZ-QUINTEIR ...
論文ID: 24-0534
発行日: 2025年
[早期公開] 公開日: 2025/09/03
ジャーナル
オープンアクセス
早期公開
Little is known about the vomeronasal organ (VNO), which is associated with social behaviors through pheromone detection in mammals, particularly ungulates. We investigated the distribution of phospholipase C beta 2 (PLCβ2), a marker of solitary chemosensory cells (SCCs), in the VNO of the Korean roe deer (Capreolus pygargus). PLCβ2-positive cells were detected in both the sensory and non-sensory epithelium of the VNO, and resembled the bipolar cells. PLCβ2-positive cells colocalized with choline acetyltransferase, and Lens culinaris agglutinin. Furthermore, PLCβ2-positive cells were sorounded with trigeminal nerver fibers with calcitonin gene-related peptide (CGRP)-positive immunoreaction. The results indicate that the VNO of the Korean roe deer exhibits a distinct distribution of SCCs, which may be involved in detecting risk factors such as harmful substances.
抄録全体を表示
-
Toshiaki TANAKA, Mika CHATANI, Asako HARAGUCHI, Makoto MATSUBAYASHI, H ...
論文ID: 25-0229
発行日: 2025年
[早期公開] 公開日: 2025/09/03
ジャーナル
オープンアクセス
早期公開
Eimeria spp. cause coccidiosis characterized by diarrhea and induce serious economic losses in livestock industries. Although several anti-coccidial drugs are currently available, the emergence of resistant strains and drug residues is problematic; therefore, the development of new drugs is needed. Since sporozoites of Eimeria spp. invade host intestinal epithelial cells and numerous merozoites are formed, drugs that target sporozoites are expected to be useful. We previously used murine Eimeria krijgsmanni as a model to examine anti-coccidial drug susceptibility; however, few studies have conducted drug evaluations against sporozoites. The establishment of excystation protocols is essential for progress in in vitro experiments using sporozoites because oocysts must be isolated from feces using complex techniques before the excystation process. Various artificial excystation protocols have been reported for each Eimeria spp.; however, those for E. krijgsmanni have not yet been examined. Therefore, 4 protocols described in previous studies were herein conducted for E. krijgsmanni. Pepsin was important for excystation in rodent Eimeria spp., and this was also the case for E. krijgsmanni. Excystation rates were higher with the physical disruption of oocyst walls than with pepsin. An incubation in HBSS containing 0.25% (w/v) trypsin and 0.1% (w/v) sodium taurocholate after a physical treatment achieved higher and the most stable excystation rates. Modifications to this method were also examined, and no improvements were observed. The optimal excystation protocol for E. krijgsmanni was elucidated as of now.
抄録全体を表示
-
Kazuki HARADA, Chisato AKITA, Hinako HARADA, Hiroki KUBOTA, Yuji SUNDE ...
論文ID: 25-0292
発行日: 2025年
[早期公開] 公開日: 2025/09/02
ジャーナル
オープンアクセス
早期公開
A 13-year-old female Persian cat with a heart murmur presented with dyspnea. Thoracic radiography revealed pleural effusion and dilation of peripheral pulmonary vessels. Echocardiography showed significant enlargement of the right atrium and ventricle and left atrial appendage; an atrial septal defect (ASD) near the atrioventricular valves; and a septum-like structure below the left atrial appendage. Left-to-right shunting through the ASD was noted, with a pulmonary-to-systemic flow ratio of 2.72. The cat was diagnosed with incomplete atrioventricular septal defect (IAVSD) and supravalvular mitral stenosis (SVMS) and subsequently medically managed for 7 months before death. Necropsy confirmed IAVSD with a mitral valve cleft and SVMS. To our knowledge, this is the longest reported survival in a cat with both anomalies.
抄録全体を表示
-
Nao KONDO, Masaaki MAEDA, Ryo MUROTSUKI, Tatsuya RYOHA, Marina OTSUKA, ...
論文ID: 25-0298
発行日: 2025年
[早期公開] 公開日: 2025/09/01
ジャーナル
オープンアクセス
早期公開
To diagnose milk fever (MF) in dairy cows under clinical practice, measurement of ionized calcium concentrations in blood using point-of-care (POC) devices is required. However, blood sampling poses a significant financial burden on farmers and increases the workload of veterinarians who must visit farms to perform the examinations. The present study aimed to evaluate whether the onset of MF could be predicted using colostrum samples, which can be collected by farmers themselves. Total calcium (tCa) concentrations in colostrum were measured using a commercially available water quality test device. The results showed that cows treated for MF had significantly higher tCa concentrations in colostrum (2,141.1 ± 623.7 mg/L) compared to cows without MF (1,546.3 ± 494.4 mg/L) (P=0.006). Receiver operating characteristic (ROC) curve analysis suggested that measuring tCa concentration in colostrum could be used to predict the onset of MF. When the cut-off value was set at >2,000 mg/L, the sensitivity and specificity were 66.7% and 88.9%, respectively. Results from these findings, measuring tCa concentration in colostrum using a water quality test device offers a simple, inexpensive, and rapid method for predicting the onset of MF in dairy cows.
抄録全体を表示
-
Kevin PONCE, Jessica JURADO, Mercy RAMIREZ, Luis VARGAS-ROCHA, Dennis ...
論文ID: 25-0183
発行日: 2025年
[早期公開] 公開日: 2025/08/29
ジャーナル
オープンアクセス
早期公開
Epizootic hemorrhagic disease virus (EHDV) is a vector-borne pathogen that affects both wild and domestic ruminants. Climate influences vector-borne diseases by driving vector migration to new areas, where they spread the virus. However, the lack of surveillance in some areas hinders accurate assessment the true disease burden. This study aimed to determine the seroprevalence of EHDV and associated risk factors in cattle from the northern region of Peru in 2022. Blood serum samples were collected from 578 cattle in the departments of Tumbes, Piura, Lambayeque, Cajamarca, and La Libertad and analyzed using cELISA. The overall EHDV seroprevalence was 17.82% (95% confidence interval 14.78–21.19). The highest proportion of seropositive animals was observed in two regions closest to the equator, Piura and Tumbes (50%), followed by Lambayeque (39.1%) and Cajamarca (11.08%). In addition, the highest seroprevalence was found at temperatures ≤20°C (50.46%), wind speeds ≤3 m/sec (31.43%), and altitudes ≤1,260 masl (37.28%), with a significant decrease at higher elevations (P<0.05). After adjusting for temperature, the odds of EHDV seropositivity were significantly lower in cattle from areas located at >2,000 to ≤3,290 masl (Odds ratio [OR]=0.15) and >3,290 (OR=0.07), compared to those from the reference altitude category of ≤1,260 masl. Similarly, after adjusting for altitude, cattle from regions with temperatures >20°C had significantly lower odds of seropositivity (OR=0.17) compared to those exposed to temperatures ≤20°C. This study reports anti-EHDV antibodies in cattle from northern Peru for the first time, highlighting associations with bioclimatic factors.
抄録全体を表示
-
Fumitaka SHITAMORI, Taisuke NAKAGAWA, Naohiro TAKAHASHI, Megumi SAKAMO ...
論文ID: 25-0152
発行日: 2025年
[早期公開] 公開日: 2025/08/28
ジャーナル
オープンアクセス
早期公開
電子付録
Peripheral nerve sheath tumors (PNSTs) originating from the vagus nerve are rare in dogs. This paper reports a canine case of malignant PNST originating from the vagus nerve, specifically the vagus-sympathetic trunk, which induced notable gagging and chronic vomiting. The clinical signs improved after surgery despite incomplete resection, and the dog achieved an overall survival of at least 514 days. These observations suggest that surgical intervention may be beneficial in dogs with malignant PNSTs originating from the vagus nerve.
抄録全体を表示
-
Hayato SUGASAWA, Saori YOSHIOKA, Youta SASAKI, Nodoka SADOSHIMA, Yuki ...
論文ID: 25-0276
発行日: 2025年
[早期公開] 公開日: 2025/08/25
ジャーナル
オープンアクセス
早期公開
Avian haemosporidian parasites cause high pathogenicity and lethal infections to non-native or introduced birds. Crested ibis (Nipponia nippon) resulted extinct in Japan but the Chinese population was introduced to Japan. Understanding the prevalence of pathogens of rare species is important for their conservation but it is often difficult to investigate them due to the limitation of the access. We collected parasite vector insects in 2022 and 2023 around the crested ibis conservation center in Sado Island. Among collected 214 mosquitoes and 585 biting midges, three Haemoproteus genetic lineages were detected from four Culicoides biting midges for the first time. Our findings suggested that the transmission of Haemoproteus could occur between biting midges and wild birds including crested ibis.
抄録全体を表示
-
Seunghoon LEE, Mi-Hwa OH, Jong-Hui KIM, Inchul CHOI, Jingu NO, Dong-Ho ...
論文ID: 25-0130
発行日: 2025年
[早期公開] 公開日: 2025/08/21
ジャーナル
オープンアクセス
早期公開
Four genetically identical cloned dogs were produced from a single donor using the somatic cell nuclear transfer technique. They stayed in the same facility during youth period, and were later assigned to different locations according to their roles as working dogs. In this study, we report observations from four genetically identical cloned working dogs exposed to different environments, including an airport and a police station, for two years. Hair and fecal samples were collected to assess potential environmental influences on mineral content and intestinal microbiota composition. The concentrations of toxic minerals, such as Pb and Ni, were significantly higher in the hair mineral analysis of the dog exposed to the airport compared to those of the three police station-working dogs. Fecal microbiota analysis showed that the intestinal microbiota of the airport-exposed dog was simplified, with an increased proportion of Actinobacteria and a decreased proportion of Lachnospiraceae and Fusobacteria. Our observations suggest that environmental conditions may influence the accumulation of harmful substances in the body and affect intestinal microbiota community. This finding also highlights the potential of non-invasive methods for evaluating workplace-related environmental influences on working dogs. These results suggest that different environments may contribute to physiological differences even among genetically identical individuals. Future studies should investigate the mechanisms underlying these variations and assess their long-term implications for canine health monitoring with larger sample sizes.
抄録全体を表示
-
Maria IZUMI, Kazuki HANO, Taiki KATO, Akihiro HIRATA, Paing THAW, Azus ...
論文ID: 25-0145
発行日: 2025年
[早期公開] 公開日: 2025/08/19
ジャーナル
オープンアクセス
早期公開
We aimed to establish a streptozotocin-induced type 1 diabetes mellitus (DM) model in Microminipigs. Initially, a dose of 150 mg/kg streptozotocin was tested, and pigs exhibited characteristic triphasic patterns in glycemic responses: phase 1 (transient hyperglycemia), phase 2 (hypoglycemia), and phase 3 (sustained hyperglycemia). Blood glucose levels showed a transient increase in phase 1, and then dropped sharply in phase 2. Based on these findings, we conducted a follow-up study using a reduced streptozotocin dose of 125 mg/kg. This group exhibited glucose dynamics similar to those observed in the 150 mg/kg group, showing glucose reductions and mild lethargy. In phase 3, both groups developed sustained hyperglycemia with negligible insulin secretion, confirming the successful establishment of a type 1 DM model.
抄録全体を表示
-
Mari YAMAMOTO, Tomohiro OKAGAWA, Satoru KONNAI, Hirohisa MEKATA
論文ID: 25-0278
発行日: 2025年
[早期公開] 公開日: 2025/08/19
ジャーナル
オープンアクセス
早期公開
Bovine immunodeficiency virus (BIV) and bovine leukemia virus (BLV) are retroviruses infecting cattle, with BLV being a known cause of enzootic bovine leukosis. In contrast, BIV is considered non-pathogenic and has been less studied, despite its relevance to animal lentiviral evolution. This study aimed to develop and validate a multiplex real-time PCR assay for simultaneous detection of BIV proviral DNA and quantification of BLV proviral load. Novel primers and probes targeting the BIV pol gene were designed. The assay demonstrated a detection limit of 10 copies per reaction with a probability of 83%, comparable to conventional nested PCR. Importantly, the addition of BIV primers and probes did not interfere with the accurate quantification of BLV. Furthermore, BIV detection was unaffected even in samples with high BLV proviral loads, confirming the assay’s robustness for simultaneous detection. The validated assay was applied to a molecular epidemiological survey of 6,051 cattle in the Kyushu region of Japan, conducted between 2021 and 2025. No BIV proviral DNA was detected in any sample, indicating a low prevalence of BIV in this population. This multiplex PCR assay provides a sensitive, efficient, and labor-saving tool for integrated surveillance of retroviral infections in cattle. Furthermore, it offers a valuable platform for future studies on the epidemiology and ecological role of BIV in bovine populations.
抄録全体を表示
-
Luli ZHOU, Jirui WANG, Maojun LIU, Guanyu HOU, Guang RONG, Renlong LV, ...
論文ID: 25-0284
発行日: 2025年
[早期公開] 公開日: 2025/08/19
ジャーナル
オープンアクセス
早期公開
Increasing antibiotic resistance in Mycoplasma hyopneumoniae (Mhp) necessitates alternative treatments. Piper sarmentosum extracts (PSE) and guava extracts (GE) show promise due to their antimicrobial and antioxidant properties. This study evaluates the combined in vitro effects of PSE and GE against Mhp and their antioxidant activities.Anti-Mhp activity was assessed using microdilution minimum inhibitory concentration (MIC) and synergy via fractional inhibitory concentration index (FICI), while antioxidant capacity was measured with 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) assays for individual extracts, and a 1:1 mixture (based on the results of FICI). Phytochemical profiling used high performance liquid chromatography (HPLC) to quantify main six bioactive compounds. HPLC showed PSE contained vitexin-2-O-rhamnoside (8.58 mg/g) and pellitorine (3.31 mg/g); GE had quercetin-3-O-glucuronide (6.85 mg/g), quercetin (3.72 mg/g), isoquercitrin (0.51 mg/g), and avicularin (1.37 mg/g). PSE (MIC 0.78 mg/mL) and GE (MIC 0.39 mg/mL) exhibited anti-Mhp effects, with their 1:1 combination being additive (FICI=0.75) and reducing MIC to 0.195 mg/mL. This mix also enhanced antioxidant activity by 9.0% in ABTS and 27.0% in FRAP assays. The 1:1 combination of PSE and GE demonstrated superior anti-Mhp activity (additive effect) and enhanced antioxidant properties (synergistic effect) compared to the individual extracts. These findings highlight the potential of these extracts as phytopharmaceutical agents.
抄録全体を表示
-
Toko MAEHARA, Atsuki FUJIMURA, Rin SEGAWA, Satoshi INOUE, Hiroshi SATO ...
論文ID: 25-0196
発行日: 2025年
[早期公開] 公開日: 2025/08/05
ジャーナル
オープンアクセス
早期公開
Prolonged lung inflammation leads to the development of asthma and approximately 27.8% of adult patients with asthma suffer from depression. We examined the effect of the prostaglandin F2α (PGF2α) receptor (FP receptor) agonist, fluprostenol, on ovalbumin (OVA)-induced asthma and asthma-related depression in mice. Repeated fluprostenol+OVA administration increased OVA-induced inflammatory cell infiltration and mRNA expression of inflammatory mediators in the lung. In contrast, in the tail suspension and forced swim tests, fluprostenol+OVA administration significantly reduced the immobile time compared with saline+OVA-administered mice. Fluprostenol+OVA treatment significantly upregulated serotonin 1A receptor and tryptophan hydroxylase in the hippocampus compared with the expression in saline+OVA mice. These results suggest that PGF receptor (FP receptor) stimulation promotes lung inflammation but attenuates depression, possibly via the serotonin pathway.
抄録全体を表示
-
Akihisa SUWA
論文ID: 24-0514
発行日: 2025年
[早期公開] 公開日: 2025/08/04
ジャーナル
オープンアクセス
早期公開
A 14-year-old, castrated male mixed-breed cat presented with weight loss and rhinitis for several months and was referred due to anemia and thrombocytopenia. A complete blood cell count (CBC) revealed severe non-regenerative anemia (hematocrit (HCT), 9.7%) and thrombocytopenia (0 /μL). Serology was positive for feline immunodeficiency virus (FIV) antibodies. Bone marrow aspiration showed moderate myeloid hyperplasia, severe erythroid hypoplasia, and megakaryocytic hypoplasia. The cat was diagnosed with pure red cell aplasia (PRCA) and amegakaryocytic thrombocytopenia (AMT) and treated with corticosteroids, leading to recovery. On day 242, bone marrow aspirates showed erythroid and megakaryocytic cell production. By day 600, both diseases had not recurred without medication. This is the documented case of concurrent PRCA and AMT in an FIV-infected cat.
抄録全体を表示
-
Otgontuya GANBAATAR, Sumiya GANZORIG, Erdene-Ochir TSEREN-OCHIR, Yasun ...
論文ID: 25-0267
発行日: 2025年
[早期公開] 公開日: 2025/08/04
ジャーナル
オープンアクセス
早期公開
In 2024, 90 soil samples and 11 fecal samples were collected from nine Mongolian provinces. Using NANAT selective agar, R. equi was successfully isolated from 23 soil samples (25.6%) across five provinces and from three fecal samples (27.3%) collected in two provinces. A total of 122 isolates were identified as R. equi via choE-targeted polymerase chain reaction (PCR) and subsequently screened for virulence-associated genes (vapA, vapB, and vapN) by PCR. Of these, 17 isolates tested positive for the vapA gene, while the remaining 105 isolates were negative for both vapB and vapN. Plasmid profiling of the vapA-positive isolates revealed the presence of an 85-kb type I virulence plasmid, which is common in isolates from Europe and North America. This is the first documented detection of vapA-positive R. equi in Mongolia.
抄録全体を表示
-
Natsuko FUKUMOTO, Hiroshi BANNAI, Nanako KAWANISHI, Makoto SHIBATA, Da ...
論文ID: 25-0271
発行日: 2025年
[早期公開] 公開日: 2025/08/04
ジャーナル
オープンアクセス
早期公開
Equine coronavirus (ECoV) causes fever, anorexia and sometimes enteric signs (e.g. diarrhea) in adult horses. Three ECoV outbreaks among draft horses at the same Japanese racecourse have been reported previously, in 2004, 2009 and 2012. We describe the epidemiological, clinical and genetic features of the fourth ECoV outbreak at the same racecourse in 2025. In January 2025, a sudden increase in the number of draft horses showing various combinations of fever, anorexia and enteric signs was observed at Obihiro Racecourse, in Hokkaido, Japan. Of about 800 horses, 176 showed clinical signs. Specifically, 83.5% (147/176) horses had fever, 98.9% (174/176) were anorexic, and 10.2% (18/176) had enteric signs. Real-time reverse transcription –polymerase chain reaction detected ECoV RNA in 23 of 26 horses. Seroconversion to ECoV was confirmed in 25 of 26 horses by neutralization assay. The results indicate that all 26 horses tested were infected with ECoV. Molecular analyses showed that the spike and nucleocapsid genes were highly conserved compared to other ECoVs. However, phylogenetic analysis showed that these genes of the 2025 virus did not form a group with the strains detected in the previous three outbreaks. These results indicate that ECoV was the cause of the sudden increase in the number of draft horses with fever, anorexia and enteric signs. Although the origin of the 2025 virus is unknown, this outbreak was likely caused by a virus different from the one that caused the previous outbreaks.
抄録全体を表示
-
Asami NISHIMORI, Shinya IWASAKI, Kiyohiko ANDOH, Yuichi MATSUURA
論文ID: 25-0109
発行日: 2025年
[早期公開] 公開日: 2025/08/01
ジャーナル
オープンアクセス
早期公開
Contamination of cell lines with bovine viral diarrhea virus (BVDV) derived from fetal bovine serum (FBS) is a major obstacle in cell biology experiments. Although the selection of BVDV-free FBS and constant viral checking are important to prevent the viral contamination of cells, they have been ineffective in the past contamination of several cell lines available from cell banks. Recently, a novel method to eliminate BVDV from one contaminated cell line, LFBK-αvβ6 cells, using BVDV inhibitor DB772 was reported. To confirm the reproducibility and possibility of practical use of this method in other cell lines, we attempted BVDV elimination using DB772 from five contaminated cell lines: BL3.1, EBTr, A26, FLK-BLV, and CC81. Treatment with DB772 significantly decreased viral genome counts in all BVDV-contaminated cells; however, cytotoxicity-like growth suppression was observed, in contrast to previously reported findings. Moreover, continuous cell passages in the presence of DB772, conducted in imitation of a previous study, resulted in the failure of elimination with rebound of viral genomes after several cell passages. Hence, we modified the method to combine limiting dilution of cultured cells with drug treatment and finally succeeded in BVDV elimination in four of the 5 cell lines, except for EBTr cells, which exhibited strong suppression of cell proliferation after DB772 treatment. Taken together, our findings will help to solve the problem of BVDV contamination in cell lines.
抄録全体を表示
-
Takumi KOMIYA, Akihiro MORI, Naohito NISHII, Hitomi ODA, Eri ONOZAWA, ...
論文ID: 2-0345
発行日: 2021年
[早期公開] 公開日: 2021/03/05
ジャーナル
オープンアクセス
早期公開
A 5-year-old castrated male domestic shorthair cat was diagnosed with diabetic ketoacidosis and severe insulin resistance. Although the conventional treatment for diabetic ketoacidosis was provided, the cat required frequent hospitalization because of severe dehydration and repeated diabetic ketoacidosis. We detected anti-insulin antibodies for human in this cat. Serum insulin-binding IgG levels were markedly elevated compared with those in healthy cats and other diabetic cats. We initiated prednisolone to suppress the effects of anti-insulin antibodies. After initiation of prednisolone, the cat was gradually recovered with increasing activity and appetite. Furthermore, satisfactory glycemic control was achieved with combined subcutaneous injection of insulin detemir and insulin degludec.
抄録全体を表示
-
Linjie LI, Xiaoling SHI, Derong ZHANG, Xin CAO, Amjad ALI, Jialin BAI
論文ID: 20-0487
発行日: 2020年
[早期公開] 公開日: 2020/11/13
ジャーナル
オープンアクセス
早期公開
-
Mario DOLERA, Luca MALFASSI, Simone PAVESI, Sara FINESSO, Giovanni MAZ ...
論文ID: 16-0077
発行日: 2017年
[早期公開] 公開日: 2017/11/15
ジャーナル
フリー
早期公開
Atlanto-axial (AA) instability due to ligament insufficiency is a common cause of cervical spinal cord compression in toy breeds. However, in some dogs a difference in size between the atlas and the axis leads to joint incongruence that exacerbates AA subluxation and makes surgical treatment challenging. Twelve dogs with AA instability with incongruence were enrolled in a single institution prospective observational study. Computed tomography (CT) and magnetic resonance imaging (MRI) of the AA joint were compared to a retrospectively reviewed control group. A novel surgical approach consisting of a dorsal internal fixation technique was performed in six dogs. For affected dogs, the mean normalised difference between the dorso-ventral atlas canal and the dorso-ventral axis canal was 29.67% (median of 35.07%, standard deviation 25.64%), while in normal dogs a mean difference of 4.67% (median of 3.95%, standard deviation 5.21%) was observed. On MRI, 12/12 affected dogs had spinal cord compression, which was classified as reducible (3/12), partially reducible (6/12) and non-reducible (3/12). In surgically operated dogs, follow-up CT showed a partial or complete reduction of the previous spinal cord compression with a consistent amelioration or resolution of the presenting complaints. The proposed surgical technique was safe and effective in dogs with partially or completely reducible spinal cord compression.
抄録全体を表示
-
Mohamed ABDO, Khaled SHOGHY, Yoshinao Z. HOSAKA, Tomohiro IMAGAWA, Saf ...
論文ID: 14-0225e
発行日: 2014年
[早期公開] 公開日: 2014/08/20
ジャーナル
フリー
早期公開
This article released online on July 30, 2014 as advance publication has been retracted by the Editorial Board of Journal of Veterinary Medical Science due to a violation of the journal’s “Information for Authors”.
抄録全体を表示
-
Mohamed ABDO, Khaled SHOGHY, Yoshinao Z. HOSAKA, Tomohiro IMAGAWA, Saf ...
論文ID: 14-0225
発行日: 2014年
[早期公開] 公開日: 2014/07/30
ジャーナル
フリー
早期公開
This article has been retracted by the Editorial Board of Journal of Veterinary Medical Science due to a violation of the journal's “Information for Authors”.
抄録全体を表示
-
論文ID: 13-0051e
発行日: 2013年
[早期公開] 公開日: 2013/11/30
ジャーナル
フリー
早期公開
This article released online on August 9, 2013 as advance publication was withdrawn from consideration for publication in The Journal of Veterinary Medical Science at author's request.
抄録全体を表示
-
Hui LI, Guoquan WU, Jiancheng LI, Shusheng TANG, Xilong XIAO, Yanan XU ...
論文ID: 13-0051
発行日: 2013年
[早期公開] 公開日: 2013/08/09
ジャーナル
フリー
早期公開
This article released online on August 9, 2013 as advance publication was withdrawn from consideration for publication in The Journal of Veterinary Medical Science at author's request.
抄録全体を表示
-
論文ID: 11-0339e
発行日: 2012年
[早期公開] 公開日: 2012/12/25
ジャーナル
フリー
早期公開
This article released online on September 26, 2012 as advance publication was withdrawn from consideration for publication in The Journal of Veterinary Medical Science at author's request.
抄録全体を表示
-
Farhad SAFARPOOR DEHKORDI
論文ID: 11-0580e
発行日: 2012年
[早期公開] 公開日: 2012/11/01
ジャーナル
フリー
早期公開
This article released online on August 3, 2012 as advance publication was withdrawn from consideration for publication in The Journal of Veterinary Medical Science at author’s request.
抄録全体を表示
-
Seyedhosein JAROLMASJED, Saeed KOLAHIAN
論文ID: 11-0339
発行日: 2012年
[早期公開] 公開日: 2012/09/26
ジャーナル
フリー
早期公開
This article released online on September 26, 2012 as advance publication was withdrawn from consideration for publication in The Journal of Veterinary Medical Science at author's request.
抄録全体を表示
-
Farhad SAFARPOOR DEHKORDI
論文ID: 11-0580
発行日: 2012年
[早期公開] 公開日: 2012/08/03
ジャーナル
フリー
早期公開
This article released online on August 3, 2012 as advance publication was withdrawn from consideration for publication in The Journal of Veterinary Medical Science at author's request.
抄録全体を表示