African clawed frog (Xenopus laevis), alien species in Japan, were seen at an irrigation pond and a drain in Sakai city in 2020. We conducted field samplings and captured 4 adults and 19 tadpoles. After the field research, we detected DNA from environmental water in the surrounding area using the method of environmental DNA (eDNA) analysis, due to confirm the distribution status of the frog. As a result, X. laevis DNA appeared at 4 sites in 18 samples from 15 water sampling sites.
