In the separation of enantiomers of chiral amines by HPLC, two different chiral stationary phases were compared using 1-arylethylamines as samples. The crown-ether based chiral stationary phase (CSP-1) showed excellent chiral separation ability for chiral 1-arylethylamines having bulky substituents on the 1-aryl group. The β- cyclodextrin type chiral stationary phase with acetylated hydroxyl group (CSP-2) was effective for chiral separation of 1-phenylethylamine and its mono-substituted