In order to develop cardiopulmonary devices for long-term use, it is important to evaluate hemolytic performance and erythrocyte trauma prior to hemolysis. Traumatized erythrocytes in those devices have the possibility of thromboembolic complication and hemolytic anemia. However, the effect of these devices on erythrocyte damage has evaluated only with free-hemoglobin concentration. In the observation of erythrocyte configuration with SEM(Scanning Electro Microscope), we found that erythrocyte configuration changed discocyte to stomatocyte prior to hemolysis during erythrocytes were subjected to shear stress. The purpose of present study is to investigate the morphological changes adapted to optical scattering density(OSD). Bovine blood sample was subjected to shear stress of 3.3 Pausing a rotational viscometer for 0, 30, 60 and 90 min and respective samples were collected. The OSD was measured around a capillary tube while He-Ne leaser(543.5nm)irradiated the flowing the blood sample in the tube. The hemolysis in the blood sample was measured by spectrometric method. In resultants, there was no increment of hemolysis in the all tests conditions. OSD was obviously increased under less than 1. 5deg of scattering angle(p<0.05)when the duration of shear stress was more than 60 min. Therefore, we considered that the OSD was affected by the morphological changes of the erythrocytes, which could be an indicator of the erythrocyte damage prior to hemolysis.
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