Synthetic methods for
13C-labeled monolignol glycosides (coniferin, syringin and p-glucocoumaryl alcohol), with the label on the α-, β-, or γ-carbon of the side chain or 4-, 5-, 3-, or 1-carbon in the aromatic ring, are described. Synthetic procedures are given for dehydrogenation polymers (DHPs) from the coniferin and
13C-enriched cell wall-DHPs using isolated tree soft parts. Experimental procedures are also described for the
13C-enrichment of specific carbon atoms of cell wall lignin by feeding the
13C-labeled coniferin to ginkgo tree shoot. A beginner’s guide to these chemical experiments is included.
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