A method is described in which cells of
Staphylococcus aureus can be converted to vesiculated large bodies of L-form. When coccal cells were incubated in a liquid growth medium containing D-cycloserine,
N-acetylmuramidase and subtilisin, a large number of vesiculated large bodies were formed. Electron microscopy revealed that development of internal vesicles arose after 6hr of incubation. When growth inhibitory concentrations of rifampicin, novobiocin, or chloramphenicol were added to the culture at 6hr of incubation, small-sized nonvesiculated bodies were produced instead of vesiculated forms. The viability of cultures was reduced by rifampicin and novobiocin but not by chloramphenicol.
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