Clay-humus complexes are stabilized by clay, humic substances and cations in soils. Since there are few studies on clay-humus complexes in the tropical and subtropical regions, it is necessary, therefore, to examine the characteristics of clay, humic substances and cations in the soils of subtropical regions. Consequently, an investigation was conducted to examine the characteristics of the humic substances, which are considered be closely related to the properties of soils cultivated with sugarcane. Soil samples were collected from 9 fields in Kita-Daito island and 14 fields in Minami-Daito island, respectively. Clay content ranged for 514.0 to 920.0 g kg
-1 in the soils of both islands, indicating that the soil texture consisted mainly of heavy clay. Kaolinite and illite were the predominant clays in the soils of both islands, while other clays included vermiculite-chlorite integrade, goethite and gibbsite. The degree of the humification of humic acids which was low was categorized as Rp type in both islands. Significant correlation coefficients were observed between the contents of exchangeable Ca and humin in the soils of Kita Daito (n=9, r=0.655*), Mimani Daito island (n=14, r=0.750**) and both islands (n=23, r=0.657*), respectively. It was suggested that the content of humin have been enhanced by the presence of exchangeable calcium in soils cultivated with sugarcane in the Kita and Minami Daito islands.
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