To establish measures against outbreak of housefly,
Musca domestica L., from organic fertilizers, inhibition effects of several agents known as stimulants to the human gustatory sense on the larval development have been studied. The emergence of 0-day-old larvae were completely inhibited, with 200mM of denatonium benzoate in water solution, 1,000mM of citric acid, 2,000mM of sodium chloride and 750mM of sucrose. The IC
50 values of these stimulants showed 35.44, 313.02, 331.39 and 218.02mM, respectively. The larval developments with denatonium benzoate and citric acid were similar to without medium. On the other hand, with sodium chloride and sucrose, the pupation and emergence rates of the 2-day-old larvae and the emergence rates of the 4-day-old larvae reduced to 14-66%, and deformed pupariums were appeared. The 0-day-old and 2-day-old larvae repelled the media with 100% inhibition concentration of the respective stimulants for the 0-day-old larvae. The most of larvae crawled out of the media with denatonium benzoate, citric acid and sodium chloride within 30 minutes, while 12-23% of the larvae stayed in the medium with sucrose. From these results, it is considered that the inhibition effect on the larval development was caused by the repellent action of denatonium benzoate and citric acid, by both of repellent action and the lethal effects of sodium chloride and sucrose.
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