We found that several BC
2F
4 lines had high leaf photosynthetic rates under light-saturated and ambient CO
2 conditions. These lines are progenies of BC
2F
1 plants with high photosynthetic capacities which were generated by backcrossing between
Oryza rufipogon (W630) and
O. sativa cv. Nipponbare, as a recurrent parent. Some photosynthetic characteristics of the BC
2F
4 lines were investigated to identify the factors increasing photosynthetic rates. Photosynthetic rates of these lines under light-saturated conditions at 50 to 700 ppm CO
2 concentrations were higher than those in Nipponbare. The estimated-maximum photosynthetic rates under light-saturated and CO
2-saturated conditions in BC
2F
4 lines were also higher than that in Nipponbare. The photosynthetic rate under light-saturated and ambient CO
2 conditions was positively correlated with the carboxylation efficiency as an indicator of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity
in vivo rather than stomatal conductance. Initial and total Rubisco activities
in vitro tended to be higher in the BC
2F
4 lines than in Nipponbare. The content of active Rubisco calculated from the activation state of Rubisco was also higher in the BC
2F
4 lines than in Nipponbare. These results suggest that high photosynthetic capacities of BC
2F
1 plants can be maintained high in their progenies and high photosynthetic rates under light-saturated and ambient CO
2 conditions in the BC
2F
4 lines are achieved mainly by the high activity of Rubisco due to the high active Rubisco content.
抄録全体を表示