Lagoon Notoro-ko is open to the Okhotsk Sea and freeze during the winter. We conducted a winter investigation into the temporal changes of particulate matters flux and chlorophyll a in sea ice and water column during the ice-covered period from February 13 to April 5, 2012. We speculated that the majority of particulate matter flux during the sea ice growth phase originated from the phytoplankton in the water column. During the transition period the majority of the sinking particulate matter was considered to be ice algae, and during the sea ice melting period, ice algae and phytoplankton contributed to an increase of sinking particulate matter.
Halophiles produce halophilic enzymes which function well in high salinity conditions. The starch binding domain（SBD）of halophilic α-amylase has been found to show typical halophilic characteristics with high aqueous solubility and folding capacity. Here we developed a fusion protein expression vector, pSBF-S1, using this SBD as a fusion partner, looking for highly soluble protein expression and efficient affinity-purification. The fusion protein expressed could be purified by dual affinity chromatographies, i.e. Ni-affinity resin with N-terminal His-tag and amylose-resin with SBD affinity. The target protein is then generated through thrombin-digestion of fusion protein at the cleavage site between the target protein and SBD.