The number of chromosomes and karyotypes in four species of the genus Semisulcospira, S. libertina, S. dolorosa, S. trachea and S. nakasekoae, were analyzed by air-dry method using gill tissue treated by colcichine, either in vivo or in vitro. Two species, S. libertina and S. trachea, which were showed to have 18 pairs of chromosomes by Burch (1968), had 18 pairs of chromosomes, together with S. dolorosa. These three species showed no differences even in karyotypes, consisting of 16 metacentric, 14 submetacentric, 4 acrocentric and 2 terocentric chromosomes. While the chromosome number of S. nakasekoae distributed only in the lake Biwa was 19 pairs of chromosomes, 2n=38, consisting of 6 metacentric, 24 submetacentric and 2 telocentric chromosomes. This much differences from what reported by Burch, 2n=26, suggest that a divergence in karyotypes may exist within the species living in the Lake Biwa.
View full abstract